DNA Sequencing

Sequencher DNA Sequencing software, funded by the Student Technology Fee, is one of the premier platforms for assembling gene fragments and analyzing DNA sequences. 

Sequencher access through HSL is limited to nine (9) simultaneous users across both Windows and Mac platforms and is only accessible on-campus or through HuskyOnNet. Licenses are dynamically allocated by the KeyServer on a “first come first serve” basis; if all licenses are in use, new incoming users can join a queue until a license opens.

Sequencher is a heavily used program, and it is not unusual for all licenses to be unavailable during peak usage hours, which are typically 9:00 a.m. to 6:00 p.m. Users who are inactive for more than 30 minutes will have their license automatically reallocated and be placed at the end of the queue if all other licenses are in use.

Service Partners

Installation

PC

  1. Download the most recent version of the KeyServer Client at Sequencher-KeyServer-Client.zip. Before you will be able to run Sequencher, you must run the KeyServer Client install files and configure them. During the KeyServer Installation process, you will be prompted to give the “Host DNS name or Address”: amos.hslib.washington.edu 
  2. Once you have entered the URL, click the “Log On” button, and a message box indicating successful connection to the KeyServer will appear. 
  3. Download the Sequencher Windows license file.
  4. To obtain Sequencher for Windows, download and/or run the Sequencher Windows installer. Upon successful installation, Sequencher will automatically connect to the KeyServer, allowing use of the program. 
  5. Remember to exit the Sequencher application when you are not actively using it due to limited number of licenses.

Mac

Note: Sequencher 5.4.6 is currently NOT COMPATIBLE with macOS Catalina (10.15). To continue using Sequencher 5.4.6 please hold off on upgrading your Mac OS at this time

  1. Download the most recent version of the KeyServer Client at Sequencher-KeyServer-Client.dmg. Before you will be able to run Sequencher®, you must run the KeyServer Client install files and configure them. During the KeyServer Installation process, you will be prompted to give the “Host DNS name or Address”: amos.hslib.washington.edu 
  2. Once you have entered the URL, click the “Log On” button, and a message box indicating successful connection to the KeyServer will appear. 
  3. Download the Sequencher Mac license file.
  4. To obtain Sequencher for Mac OSX, download and/or run the Sequencher Mac installer. Upon successful installation, Sequencher will automatically connect to the KeyServer, allowing use of the program. 
  5. Remember to exit the Sequencher application when you are not actively using it due to limited number of licenses. 

This service is available to researchers in Washington, Wyoming, Alaska, Montana, and Idaho.